Fulvic Acid, Amino Acids, and Vermicompost Enhanced Yield and Improved Nutrient Profile of Soilless Iceberg Lettuce

Abstract

Soilless cultivation systems are sustainable innovations in modern agriculture, promoting high efficiency per unit area, supporting food sustainability, and addressing the growing demand for high-quality produce with minimal environmental impact. This study evaluates the effects of fulvic acid, amino acid, and vermicompost biostimulants on the growth, yield, and nutrient profile of soilless-grown iceberg lettuce (Lactuca sativa var. capitata) in floating culture under controlled glasshouse conditions. Two experiments were conducted to determine the most effective concentrations and combinations of biostimulants. In the first experiment, varying doses of fulvic acid (40 and 80 ppm), amino acid (75 and 100 ppm), and vermicompost (1 and 2 mL L-1) were tested alongside a control. Optimal doses were identified based on their positive effects on lettuce growth and yield. The second experiment examined combinations of fulvic acid, amino acid, and vermicompost extract compared to a control. Biostimulants improved lettuce growth, nutrient uptake, and antioxidants. Vermicompost boosted root biomass and leaf area, while fulvic acid and amino acid reduced nitrates and increased dry matter. Fulvic acid and vermicompost resulted in the highest yield (17.15 kg/m2, 18.2% increase), and the combined treatment maximized antioxidants, increasing vitamin C by 17.16%, total phenols by 52.54%, and flavonoids by 52.38%. These findings highlight the potential of biostimulants as eco-friendly solutions for optimizing lettuce production in soilless systems.